On the Reconstitution of the Crystalline Components of the Sea Urchin Fertilization Membrane
نویسنده
چکیده
The characteristics of the reconstitution of a crystalline component of the sea urchin fertilization membrane are presented. The reassembly of large aggregates of cylindrical or tubular components is effected by the addition of calcium or other divalent cations. The reassembly requires a slightly alkaline pH and is little affected by increasing ionic strength. Reassembly is strongly inhibited by treatment with reducing agents such as dithiothreitol. The role of this protein in the formation of the fertilization membrane and its possible relation to the calcium-insoluble proteins of the mitotic apparatus are discussed.
منابع مشابه
Determination of developmental stages of embryo in the Sea Urchin, Echinometra mathaei
Sea Urchin is one of the most useful tools in developmental biology studies because this organism has the simplest kind of developmental stages. We aimed to determine developmental stages and timetable of Echinometra mathaei embryo (the species of Persian Gulf). The spawning of E. mathaei was induced by 0.5M KCl injection (1ml) into the coelomic cavity. After fertilization, embryos were placed ...
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عنوان ژورنال:
- The Journal of Cell Biology
دوره 45 شماره
صفحات -
تاریخ انتشار 1970